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Testing Cell Respiration and Alcoholic Fermentation

There will be a measurement of heat generation produced by seedlings during cell respiration. Using a thermometer, the heat of seedlings in a thermos bottle will be calculated over a period of at least 25 minutes in 5-minute increments. Using indicator TTC you will also be able to distinguish viable seeds. Along with studies of cell respiration through temperature readings there will also be investigation of alcoholic fermentation in yeast. A respirometer will be constructed for the ability of recording the effects of yeast concentration on the rate of fermentation. By doing this, the CO2 evolved can be measured over a 20 minute time period. There will be four different tubes filled with four different yeast concentrations for the comparison of rates.

The energy yielding metabolism function in which oxygen is used is known as cell respiration. The chemical expression for cell respiration is: C6H12O6 + 6O2 yields to 6CO2 + 6H2O + Chemical Energy. Aerobic cell respiration is the metabolic pathway that requires oxygen. An example of this is glucose metabolism, which can be divided into three parts.
Thefirst part is glycolysis, which is the breakdown of glucose and substrate level phosphorylation and the formation of energy products. During glycolysis, glucose enters the pathway and gets phosphorylated by ATP twice. Once it is phosphorylated the glucose splits into tow 3-carbon fuels that are later oxidized by enzymes and phosphorylated.During the substrate-level phosphorylation 4 ATP are produced. However the end products of glycolysis are 2 ATP, 2 NADH, and 2 molecules of pyruvate.
The second part of glucose metabolism is the citric acid cycle. This is the complete oxidation of glycolytic products to CO2 and H2O. The products that are produced are protons and electrons. With each turn of the cycle there is use of oxaloacetate. The oxaloacetate joins with acetyl-CoA and when water enters the coenzyme A is released which forms ci…

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