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Dissection and Isolation of a frogs CNS

Dissection of an adult bullfrog’s central nervous system (CNS)
Experiment consists of the dissection and analyzation of a bullfrog;s nervous system.Dissection consists of the isolation of the CNS consisting of the brain and spinal cord.It also consists of analyzing the nervous tissue under the microscope.
Materials needed:frog, scalpel, razor blade, dissecting tray, pins, forceps, scissors, microscope, slides, slide covers, water, blue dye, diagram of frog;s brain, paper towel.
The bullfrog;s CNS is composed of the brain, which is further divided into the olfactory bulb, cerebrum, optic tract, optic lobe, pituitary gland, and the cerebellum.Also contained within the CNS is the spinal cord which in contained within the spineThe brain was about an inch in length and ? of an inch in diameter.The spinal cord was about 2.5 inches in length and the diameter of the spine was about 1/8 of an inch.The cerebellum in the frogs brain is very small in comparison to the rest of the brain and also to the size of the cerebellum in other animals compared to the size of their brains.
Place the frog in the dissecting tray so the ventral section faces upwards with the head pointing away from you.This will allow for easier access to the CNS since you don;t have to cut through the skull. Pin each of its legs down to the tray using the pins. Using the scalpel start the incision at the base of the jaw.This will be a superficial cut just enough to cut through the skin.Pull the scalpel in a caudal direction towards the tail.After you;ve done this make another incision lateral to where you started thefirst one and another one where you ended the cut.Pull the skin back and either cut it off or pin it down.Muscle of the breastbone and abdomen will be exposed.Using the scissors make a deep cut through this, again cutting in a caudal direction to the tail. Cut off the arms of the bullfrog and r

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