Tiffani May Chemistry Period 2
Biotin is important for healthy hair and skin. 100 mg of biotin may prevent hair loss in some men. Biotin helps to relieve muscle pain. It promotes healthy nerve tissue, bone marrow and sweat glands. It also relieves seborrheic dermatitis in infants.
Biotin works with folic acid and vitamin B12 to break down fats, protein, and carbohydrates. Biotin is found in most foods and also manufactured by bacteria in the intestinal tract. Most biotin deficiencies are associated with the consumption of raw egg whites which contain avidin. Avidin binds with biotin to prevent its absorption into the blood. Cooking the egg whites deactivates avidin. Biotin is non-toxic and probably not required in supplement form. Although biotin deficiencies are rare, they can occur when people have malabsorption problems. Some research indicates that male pattern baldness may respond to supplemented biotin, albeit in a very marginal fashion.
Biotin is found in cooked egg yolks, salt-water fish, beef and poultry, milk, cheese, soy products and whole grain breads. If athletes are eating raw egg whites, extra biotin must be consumed.
Reagent for enzymatic incorporation of biotin into nucleic acids. Biotin-21-dUTP is a dTTP analog with biotin covalently attached to the pyrimidine ring through a 21-atom spacer arm. It can be incorporated by nick translation, mixed primer labeling, 3′-end-labeling, or PCR. Biotin-21-dUTP has a 21-atom spacer arm, the longest spacer available, which reduces steric hindrance in subsequent detection using streptavidin-conjugated enzymes. 100 µl is sufficient for 50 nick translation reactions. Biotin-21-dUTP is provided with a complete User Manual (PT1464-1).
Biotin-21-dUTP (0.5 mM) 100 µl 5021-1
Biotin-21-dUTP (10 mM) 100 µl 5021-3
100 µl of 0.5 mM solution in 50 mM Tris-HCl (pH 7.5)

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