bio lab 6

Microscopy and the Electron Microscope
Humans have been magnifying objects for centuries by using lens. Using lens to magnify objects has helped mankind know more about the cell theory and ways of life. Zacharias constructed thefirst compound microscope. Robert Hooke used the microscope to examine slices of cork. He noticed many "little boxes" and called them cells. Cells are the building blocks of life. We would not know as much about cells as we do with out microscopes. It is important to know about the different types of microscopes and how they function.
CD player, Individualized Biology lab book, pen, and your brain. Objectives:
(See page 2 ofIndividualizedBiology Unit 9 Script.)
Anton van Leeuwenhoek is considered to be the father of bacteriology. He used a simple microscope consisting of one lens to observe bacteria and other unicellular microorganisms. He grinded the lens he used, so that the lens had great curvature. He reached magnifications of 270 diameters. This was the most reached from his time. Magnification increases the visibility of what ever detail the objective lens can reveal. There is a significant relationship between wave length and resolving power. Wavelength is the distance between corresponding points on two successive waves. The shorter the wave lengths are used, the greater the resolving power is. Two objects that are closer together than one half the wave length of light used can not be seen as separate objects. There are several ways to increase the resolving power. You can use a shorter wave length, project on to screens, use quartz lens, use ultraviolet light, or use an objective lens that can accept a greater cone of light. The wavelength that would give the best resolving power for a light microscope is blue. Light source has a great effect on a microscope. The bright fi

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